Darryl Pappin

Identification of myocardial proteins from two‐dimensional gels by peptide mass fingerprinting

Abstract Two‐dimensional gels offer a powerful method for separating complex protein mixtures, but subsequent methods for analysing individual components, such as protein sequen...

Probability-based protein identification by searching sequence databases using mass spectrometry data

Several algorithms have been described in the literature for protein identification by searching a sequence database using mass spectrometry data. In some approaches, the experi...

Rapid identification of proteins by peptide-mass fingerprinting

A human DNA editing enzyme homologous to the Escherichia coli DnaQ/MutD protein

Re‐evaluation of the primary structure of <i>Ralstonia eutropha</i> phasin and implications for polyhydroxyalkanoic acid granule binding

Sequence analysis of several cDNAs encoding the phasin protein of Ralstonia eutropha indicated that the carboxyl terminus of the resulting derived protein sequence is different ...

Chemistry, Mass Spectrometry and Peptide-Mass Databases: Evolution of Methods for the Rapid Identification and Mapping of Cellular Proteins

Large-format 2D gel electrophoresis systems have been developed that are capable of resolving several thousand cellular proteins in a matter of days [1,2]. For a number of years...