Richard A. Keller

Detection of single fluorescent molecules

Alterations of Single Molecule Fluorescence Lifetimes in Near-Field Optical Microscopy

Fluorescence lifetimes of single Rhodamine 6G molecules on silica surfaces were measured with pulsed laser excitation, time-correlated single photon counting, and near-field sca...

Single molecule detection and photochemistry on a surface using near-field optical excitation

We report the first photobleaching experiments of single molecules on a surface. Single molecules of rhodamine 6G are detected on a silica surface under ambient conditions using...

Single-Molecule Fluorescence Analysis in Solution

Over the past five years, several groups have developed the capability to detect and identify single fluorescent molecules in solution as the molecules flow through a focused la...

High-Speed DNA Sequencing: An Approach Based Upon Fluorescence Detection of Single Molecules

We are developing a laser based technique for the rapid sequencing of large fragments (approximately 40 kb) of DNA based upon the detection of single, fluorescently tagged nucle...