3′ UTR seed matches, but not overall identity, are associated with RNAi off-targets

Keywords

RNA interferenceSmall interfering RNAGene silencingBiologyGeneUntranslated regionComputational biologyGeneticsTrans-acting siRNARandom hexamerGene expressionRNA silencingThree prime untranslated regionTransfectionRNACell biologyMolecular biology

Affiliated Institutions

Agilent Technologies (United States) US

Related Publications

Experimental validation of the importance of seed complement frequency to siRNA specificity

Emily M. Anderson , Amanda Birmingham , Scott Baskerville +6 more

Pairing between the hexamer seed region of a small interfering RNA (siRNA) guide strand (nucleotides 2–7) and complementary sequences in the 3′ UTR of mature transcripts has bee...

2008 RNA 144 citations

Systematic Identification of mRNAs Recruited to Argonaute 2 by Specific microRNAs and Corresponding Changes in Transcript Abundance

David G. Hendrickson , Daniel J. Hogan , Daniel Herschlag +2 more

microRNAs (miRNAs) are small non-coding RNAs that regulate mRNA stability and translation through the action of the RNAi-induced silencing complex (RISC). Our current understand...

2008 PLoS ONE 171 citations

Determinants of targeting by endogenous and exogenous microRNAs and siRNAs

Cydney Nielsen , Noam Shomron , Rickard Sandberg +3 more

Vertebrate mRNAs are frequently targeted for post-transcriptional repression by microRNAs (miRNAs) through mechanisms involving pairing of 3′ UTR seed matches to bases at the 5′...

2007 RNA 384 citations

A microRNA in a Multiple-Turnover RNAi Enzyme Complex

György Hutvágner , Phillip D. Zamore

In animals, the double-stranded RNA-specific endonuclease Dicer produces two classes of functionally distinct, tiny RNAs: microRNAs (miRNAs) and small interfering RNAs (siRNAs)....

2002 Science 2048 citations

A 3′-Untranslated Region (3′UTR) Induces Organ Adhesion by Regulating miR-199a* Functions

Daniel Y. Lee , Tatiana Shatseva , Zina Jeyapalan +3 more

Mature microRNAs (miRNAs) are single-stranded RNAs of 18-24 nucleotides that repress post-transcriptional gene expression. However, it is unknown whether the functions of mature...

2009 PLoS ONE 98 citations

Publication Info

Year: 2006
Type: article
Volume: 3
Issue: 3
Pages: 199-204
Citations: 873
Access: Closed

External Links

View on DOI.org

Social Impact

Altmetric

3′ UTR seed matches, but not overall identity, are associated with RNAi off-targets

PlumX Metrics

Social media, news, blog, policy document mentions

Citation Metrics

873

OpenAlex

Cite This

APA Style

                            
                                
                                    Amanda Birmingham, 
                                
                                    Emily M. Anderson, 
                                
                                    Angela Reynolds
                                
                                et al.
                            
                            (2006). 
                            3′ UTR seed matches, but not overall identity, are associated with RNAi off-targets. 
                            Nature Methods
                            , 3
                            (3)
                            , 199-204.
                            https://doi.org/10.1038/nmeth854
                        

Identifiers

DOI: 10.1038/nmeth854