A neutralizing human antibody binds to the N-terminal domain of the Spike protein of SARS-CoV-2

Abstract

Hitting SARS-CoV-2 in a new spot A key target for therapeutic antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the spike protein, a trimeric protein complex with each monomer comprising an S1 and an S2 domain that mediate binding to host cells and membrane fusion, respectively. In addition to the receptor binding domain (RBD), S1 has an N-terminal domain (NTD). In searching for neutralizing antibodies, there has been a focus on the RBD. Chi et al. isolated antibodies from 10 convalescent patients and identified an antibody that potently neutralizes the virus but does not bind the RBD. Cryo–electron microscopy revealed the epitope as the NTD. This NTD-targeting antibody may be useful to combine with RBD-targeting antibodies in therapeutic cocktails. Science , this issue p. 650

Keywords

Spike ProteinSpike (software development)Terminal (telecommunication)AntibodySevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)VirologyCoronavirus disease 2019 (COVID-19)ChemistryBiologyComputer scienceImmunologyMedicine

MeSH Terms

AdultAngiotensin-Converting Enzyme 2AnimalsAntibodiesMonoclonalAntibodiesNeutralizingAntibodiesViralAntibody AffinityAntibody SpecificityAntigensViralB-LymphocytesBetacoronavirusCOVID-19Chlorocebus aethiopsCoronavirus InfectionsCoronavirus Nucleocapsid ProteinsCryoelectron MicroscopyEnzyme-Linked Immunosorbent AssayGenesImmunoglobulin Heavy ChainHumansImmunologic MemoryMiddle AgedMutationNucleocapsid ProteinsPandemicsPeptidyl-Dipeptidase APhosphoproteinsPneumoniaViralProtein DomainsProtein Interaction Domains and MotifsReceptorsCoronavirusReceptorsVirusSARS-CoV-2Spike GlycoproteinCoronavirusVero CellsYoung Adult

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Publication Info

Year: 2020
Type: article
Volume: 369
Issue: 6504
Pages: 650-655
Citations: 1543
Access: Closed

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APA Style

                            
                                
                                    Xiangyang Chi, 
                                
                                    Renhong Yan, 
                                
                                    Jun Zhang
                                
                                et al.
                            
                            (2020). 
                            A neutralizing human antibody binds to the N-terminal domain of the Spike protein of SARS-CoV-2. 
                            Science
                            , 369
                            (6504)
                            , 650-655.
                            https://doi.org/10.1126/science.abc6952
                        

Identifiers

DOI: 10.1126/science.abc6952
PMID: 32571838
PMCID: PMC7319273

Data Quality

Data completeness: 90%