A Protein Interaction Map of Drosophila melanogaster

Abstract

Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid–based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence was developed to refine this draft map to a higher confidence map of 4679 proteins and 4780 interactions. Statistical modeling of the network showed two levels of organization: a short-range organization, presumably corresponding to multiprotein complexes, and a more global organization, presumably corresponding to intercomplex connections. The network recapitulated known pathways, extended pathways, and uncovered previously unknown pathway components. This map serves as a starting point for a systems biology modeling of multicellular organisms, including humans.

Keywords

Drosophila melanogasterComputational biologyBiologyProteomeMulticellular organismModel organismDrosophila (subgenus)Multiprotein complexMelanogasterSystems biologyGeneticsGene

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Publication Info

Year: 2003
Type: article
Volume: 302
Issue: 5651
Pages: 1727-1736
Citations: 2270
Access: Closed

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APA Style

                            
                                
                                    Loïc Giot, 
                                
                                    Joel S. Bader, 
                                
                                    Cory Brouwer
                                
                                et al.
                            
                            (2003). 
                            A Protein Interaction Map of <i>Drosophila melanogaster</i>. 
                            Science
                            , 302
                            (5651)
                            , 1727-1736.
                            https://doi.org/10.1126/science.1090289
                        

Identifiers

DOI: 10.1126/science.1090289