Abstract
Abstract A simple, sensitive silver staining method for sodium dodecyl sulfate polyacrylamide gel electrophoresis has been developed which yields high resolution of proteins with a transparent background. The method includes the incorporation of a water wash after a short fixation step in ethanol/acetic acid and prior to glutaraldehyde cross‐linking, which appears to be necessary for the high resolution staining of protein bands and the low background staining.
Keywords
Silver stainSodium dodecyl sulfateStainingPolyacrylamideChromatographyChemistryAcetic acidPolyacrylamide gel electrophoresisGlutaraldehydeStainGel electrophoresisSodiumResolution (logic)Gel electrophoresis of proteinsBiochemistryMolecular biologyBiologyEnzymePolymer chemistryOrganic chemistry
MeSH Terms
ElectrophoresisPolyacrylamide GelPlant ProteinsRhodophytaSensitivity and SpecificitySilver Staining
Affiliated Institutions
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Publication Info
- Year
- 1995
- Type
- article
- Volume
- 16
- Issue
- 1
- Pages
- 948-951
- Citations
- 135
- Access
- Closed
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Cite This
Marianne Swain,
Neil W. Ross
(1995).
A silver stain protocol for proteins yielding high resolution and transparent background in sodium dodecyl sulfate‐polyacrylamide gels.
Electrophoresis
, 16
(1)
, 948-951.
https://doi.org/10.1002/elps.11501601159
Identifiers
- DOI
- 10.1002/elps.11501601159
- PMID
- 7498141