Abstract

A simple technique for the determination in vitro of the dry‐ or organic‐matter digestibility of small (0·5 g) samples of dried forages is described. It involves incubation first with rumen liquor and then with acid pepsin. Using 146 samples of grass, clover and lucerne of known in vivo digestibility ( Y ), the regression equation Y = 0·99 X– 1·01 (S.E. ± 2·31) has been calculated, where X = in vitro dry‐matter digestibility. This technique has been used for the study of the digestibilities of plant breeder's material, of the leaf and stem fractions of herbage and of herbage consumed by animals.

Keywords

Dry matterForageRumenDigestion (alchemy)IncubationAgronomyOrganic matterAnimal scienceBiologyIn vitroPepsinChemistryFood scienceBiochemistryChromatographyFermentationEnzymeEcology

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Tilley et al . (1) have clearly demonstrated that their rumen liquor and pepsin procedure for the determination of digestibility of herbage is useful and precise. The method has...

1961 Grass and Forage Science 39 citations

Publication Info

Year
1963
Type
article
Volume
18
Issue
2
Pages
104-111
Citations
6494
Access
Closed

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J. M. A. Tilley, R. A. Terry (1963). A TWO‐STAGE TECHNIQUE FOR THE <i>IN VITRO</i> DIGESTION OF FORAGE CROPS. Grass and Forage Science , 18 (2) , 104-111. https://doi.org/10.1111/j.1365-2494.1963.tb00335.x

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DOI
10.1111/j.1365-2494.1963.tb00335.x