Actinobacteria with heavy metal tolerance from the Sinop coast: A molecular characterization approac

Abstract

This study aimed to isolate and molecularly characterize marine actinobacteria tolerant to heavy metals from sediment samples collected from the Meydankapı coast in Sinop, Türkiye. Sediment samples were obtained from three different stations and cultured on five selective media, leading to the isolation of 54 actinobacteria strains. The highest isolation efficiency was achieved on R2A and SM3 media. The tolerance of the isolates to heavy metals, including lead (Pb), copper (Cu), zinc (Zn), mercury (Hg), and cadmium (Cd), was tested. The results revealed that all isolates were sensitive to Hg and Cd but exhibited high tolerance to Pb. Twelve isolates, showing the highest resistance to heavy metals, were selected for genomic DNA isolation. The 16S rRNA gene region was amplified using universal primers and sequenced. Sequence analysis identified the isolates as belonging to the genera Streptomyces, Actinomadura, Nocardia and Nonomuraea. The approximately 1500-bp 16S rRNA gene sequences were compared with the EzTaxon database, and the closest species were determined. The obtained sequences were deposited in the GenBank database. The results indicate that marine actinobacteria isolated from polluted sediments of the Black Sea possess significant potential for bioremediation and environmental biotechnology applications due to their resistance mechanisms to heavy metals.

Affiliated Institutions

• Hitit Üniversitesi TR
• Sinop University TR
• Çankırı Karatekin University TR

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