Activated Carbons for Bone Cell Growth: Structural Properties and Biological Interactions

Abstract

Having high porosity and biocompatibility, carbon-based materials are promising candidates for tissue engineering applications, particularly as substitutes for biological tissues. This study investigates the growth and viability of osteoblasts on four different activated carbon (AC) materials and correlates biological responses with their physicochemical and morphological properties. Two materials derived from non-renewable sources—AC1, a laboratory-synthesized carbon derived from anthracite, and AC3, a commercial activated carbon (Norit GCN 830) derived from coal—and two commercial activated carbons derived from renewable sources—peat, AC2 (Norit PK1-3), and wood, AC4 (ROX 0.8)—are studied. Results showed that AC1 exhibited the highest porosity (3072 m2/g), with higher phenolic and oxygen-containing surface groups but lower cell viability. In contrast, AC2, AC3, and AC4 displayed lower porosity compared to AC1 (755, 1040, and 1083 m2/g, respectively) and fewer surface phenolic groups but sustained osteoblast proliferation. Notably, AC4 demonstrated superior performance, characterized by regions of fibrous surface, pores in the meso- and microscale range (<50 nm), and enhanced cell viability and proliferation. AC2 also showed favorable results, ranking second for cell growth support. These findings suggest that biomass-derived ACs, particularly AC4 and AC2, provide favorable environments for osteoblast viability and proliferation. AC costs were estimated at 15 to 38 times lower than those for hydroxyapatite and bioceramics, which are widely used for bone cell growth. Thus, ACs made from renewable sources are promising candidates for tissue engineering applications, offering sustainable and effective alternatives for biomedical use.

Affiliated Institutions

• National Institute for Space Research BR
• Centre National de la Recherche Scientifique FR
• Institut Jean Lamour FR
• Université du Québec en Abitibi-Témiscamingue CA
• Université de Lorraine FR
• Universidade de São Paulo BR
• Institut Universitaire de France FR
• Universidade Nove de Julho BR

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