An automated enzymic micromethod for the measurement of fat in human milk

Abstract

Summary Most (98%) of the fat in human milk is present as triglycerides. This paper describes the use of a clarification procedure that enables the level of human milk fat to be determined by measurement of glycerol released by enzymic hydrolysis of triglycerides. The method requires only 10–50 μl milk, thus presenting a possible technique for work with small mammals, and is suitable for use with autoanalysers, permitting rapid sample throughput.

Keywords

Food scienceMilk fatChromatographyChemistry

Affiliated Institutions

Medical Research Council GB

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Publication Info

Year: 1987
Type: article
Volume: 54
Issue: 4
Pages: 487-492
Citations: 12
Access: Closed

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APA Style

                            
                                
                                    Alan Lucas, 
                                
                                    Geoffrey J. Hudson, 
                                
                                    Peter Simpson
                                
                                et al.
                            
                            (1987). 
                            An automated enzymic micromethod for the measurement of fat in human milk. 
                            Journal of Dairy Research
                            , 54
                            (4)
                            , 487-492.
                            https://doi.org/10.1017/s0022029900025693
                        

Identifiers

DOI: 10.1017/s0022029900025693