Abstract
By means of agarose-gel electrophoresis, endonuclease R· Eco RI-generated fragments of DNA from various viruses were separated, their molecular weights were determined, and complete or partial fragment maps for lambda, φ80, and hybrid phages were constructed.
Keywords
Affiliated Institutions
Related Publications
Estimation of DNA sequence divergence from comparison of restriction endonuclease digests
An estimation of the DNA sequence divergence between defined DNA secquences of individuals or species may be made from comparison by gel electrophoresis of restriction endonucle...
Human mitochondrial DNA: analysis of 7S DNA from the origin of replication.
Heat-treated samples of human mitochondrial DNA (mtDNA) exhibited a set of three low molecular weight DNA bands in addition to the major mtDNA band when electrophoresed in polya...
Properties of overexpressed phage T5 D15 exonuclease. Similarities with Escherichia coli DNA polymerase I 5‘-3‘ exonuclease.
The D15 gene of the bacteriophage T5, thought to encode an exonuclease, was cloned into an M13 phage on a 1344-base pair fragment. The deduced amino acid sequence of 291 residue...
Specific cleavage analysis of mammalian mitochondrial DNA.
Mitochondrial DNA from several mammalian species has been digested with a site-specific restriction endonuclease (HaeIII) from Haemophilus aegyptius. A quantitative analysis of ...
beta-Glucuronidase from Escherichia coli as a gene-fusion marker.
We have developed a gene-fusion system based on the Escherichia coli beta-glucuronidase gene (uidA). The uidA gene has been cloned from E. coli K-12 and its entire nucleotide se...
Publication Info
- Year
- 1974
- Type
- article
- Volume
- 14
- Issue
- 5
- Pages
- 1235-1244
- Citations
- 632
- Access
- Closed
External Links
Social Impact
Social media, news, blog, policy document mentions
Citation Metrics
Cite This
Identifiers
- DOI
- 10.1128/jvi.14.5.1235-1244.1974