Assay optimization for molecular detection of Zika virus

Abstract

We provide reagents and updated protocols for Zika virus detection suitable for the current outbreak strains. Some published assays might be unsuitable for Zika virus detection, due to the limited sensitivity and potential incompatibility with some strains. Viral concentrations in the clinical samples were close to the technical detection limit, suggesting that the use of insensitive assays will cause false-negative results.

Keywords

Zika virusVirologyBiologyComputational biologyMedicineVirus

MeSH Terms

HumansReal-Time Polymerase Chain ReactionSensitivity and SpecificityZika Virus Infection

Affiliated Institutions

Related Publications

Zika Virus Infection in Dexamethasone-immunosuppressed Mice Demonstrating Disseminated Infection with Multi-organ Involvement Including Orchitis Effectively Treated by Recombinant Type I Interferons

Jasper Fuk‐Woo Chan , Jinxia Zhang , Chris Chung‐Sing Chan +15 more

Besides virus dissemination, inflammation of various tissues, especially orchitis, may be potential complications of ZIKV infection with significant implications on disease tran...

2016 EBioMedicine 116 citations

[The situation of dengue in the world].

F Rodhain

The situation of dengue in the world can be summarized as follows: 2,5 billions persons at risk, 60 millions cases per year, and 30 000 deaths per year. The four dengue serotype...

1996 PubMed 28 citations

Dengue and Dengue Hemorrhagic Fever

Duane J. Gubler

SUMMARY Dengue fever, a very old disease, has reemerged in the past 20 years with an expanded geographic distribution of both the viruses and the mosquito vectors, increased epi...

1998 Clinical Microbiology Reviews 4279 citations

Species-Diagnostic Differences in a Ribosomal DNA Internal Transcribed Spacer from the Sibling Species Anopheles Freeborni and Anopheles Hermsi (Diptera:Culicidae)

Charles H. Porter , F. H. Collins

Approximately 460 base pairs (bp) of DNA sequence that included the second internal transcribed spacer (ITS2) and some flanking 5.8S and 28S ribosomal RNA coding regions were co...

1991 American Journal of Tropical Medicine... 282 citations

Evaluation of NxTAG Respiratory Pathogen Panel and Comparison with xTAG Respiratory Viral Panel Fast v2 and Film Array Respiratory Panel for Detecting Respiratory Pathogens in Nasopharyngeal Aspirates and Swine/Avian-Origin Influenza A Subtypes in Culture Isolates

Kwok Hung Chan , Kelvin Kai‐Wang To , P. T. W. Li +9 more

This study evaluated a new multiplex kit, Luminex NxTAG Respiratory Pathogen Panel, for respiratory pathogens and compared it with xTAG RVP Fast v2 and FilmArray Respiratory Pan...

2017 Advances in Virology 18 citations

Publication Info

Year: 2016
Type: article
Volume: 94
Issue: 12
Pages: 880-892
Citations: 160
Access: Closed

External Links

Download PDF (Free) View on DOI.org PubMed Semantic Scholar

Social Impact

Altmetric

Assay optimization for molecular detection of Zika virus

PlumX Metrics

Social media, news, blog, policy document mentions

Citation Metrics

160

OpenAlex

Influential

140

CrossRef

Cite This

APA Style

                            
                                
                                    Victor M. Corman, 
                                
                                    Andrea Rasche, 
                                
                                    Cécile Baronti
                                
                                et al.
                            
                            (2016). 
                            Assay optimization for molecular detection of Zika virus. 
                            Bulletin of the World Health Organization
                            , 94
                            (12)
                            , 880-892.
                            https://doi.org/10.2471/blt.16.175950
                        

Identifiers

DOI: 10.2471/blt.16.175950
PMID: 27994281
PMCID: PMC5153932

Data Quality

Data completeness: 86%