Abstract

An ongoing theme of the COVID-19 pandemic is the need for widespread availability of accurate and efficient diagnostic testing for detection of SARS-CoV-2 and antiviral antibodies in infected individuals. This report describes various assay techniques and tests for COVID-19 diagnosis. Most tests for early detection of SARS-CoV-2 RNA rely on the reverse transcription-polymerase chain reaction, but isothermal nucleic acid amplification assays, including transcription-mediated amplification and CRISPR-based methodologies, are promising alternatives. Identification of individuals who have developed antibodies to the SARS-CoV-2 virus requires serological tests, including enzyme-linked immunosorbent assay (ELISA) and lateral flow immunoassay. This report also provides an overview of current development in COVID-19 diagnostic techniques and products to facilitate future improvement and innovation.

Keywords

Coronavirus disease 2019 (COVID-19)Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)VirologyReverse Transcription Loop-mediated Isothermal AmplificationImmunoassayComputational biologyDiagnostic testSerologyPolymerase chain reactionReverse transcription polymerase chain reaction2019-20 coronavirus outbreakPandemicBiologyReverse transcriptaseAntibodyMedicineInfectious disease (medical specialty)ImmunologyDiseaseGeneGeneticsMessenger RNAPathology

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Year
2020
Type
article
Volume
6
Issue
5
Pages
591-605
Citations
999
Access
Closed

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Cite This

Linda J. Carter, L. Garner, Jeffrey Smoot et al. (2020). Assay Techniques and Test Development for COVID-19 Diagnosis. ACS Central Science , 6 (5) , 591-605. https://doi.org/10.1021/acscentsci.0c00501

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DOI
10.1021/acscentsci.0c00501