Abstract
Abstract An automated colorimetric procedure is described for the determination of serum and urine uric acid with improved specificity. Hydrogen peroxide, formed when uric acid is oxidized by uricase, oxidatively couples with 3-methyl-2-benzothiazolinone hydrazone and N,N-dimethylaniline, in the presence of peroxidase, to produce a stable, sensitive indamine dye. The method is reproducible, and the results correlate well with those obtained by a manual spectrophotometric uricase procedure. Also, the automated procedure is resistant to many added reducing substances that cause positive interferences in alkaline phosphotungstate methods.
Keywords
Uric acidChemistryHydrogen peroxidePeroxidaseHydrazoneUrate oxidaseChromatographyDimethylanilineUrinePeroxideColorimetryBiochemistryNuclear chemistryOrganic chemistryEnzyme
Affiliated Institutions
Related Publications
Contemporary Prevalence of Gout and Hyperuricemia in the United States and Decadal Trends: The National Health and Nutrition Examination Survey, 2007–2016
Objective To estimate the current prevalence rates and decadal trends of gout and hyperuricemia in the US, as well as the prevalence of urate‐lowering therapy (ULT) among gout p...
Gout-associated uric acid crystals activate the NALP3 inflammasome
Development of the acute and chronic inflammatory responses known as gout and pseudogout are associated with the deposition of monosodium urate (MSU) or calcium pyrophosphate di...
Treatment of Hypertension
The majority of deaths attributable to hypertension are coronary artery disease (CAD) deaths and, consequently, the prevention of CAD should be the primary aim of hypertension m...
Prevalence and Risk Factors of Nonalcoholic Fatty Liver Disease in an Adult Population of Taiwan: Metabolic Significance of Nonalcoholic Fatty Liver Disease in Nonobese Adults
Background The prevalence of nonalcoholic fatty liver disease (NAFLD) is rarely reported in Taiwan. Goals To determine the prevalence and risk factors of NAFLD in an adult popul...
Serum uric acid levels and non-alcoholic fatty liver disease in Uyghur and Han ethnic groups in northwestern China
OBJECTIVE: The aim of this study was to compare serum uric acid values in two ethnically distinct Chinese populations: Uyghur, with a high prevalence of nonalcoholic fatty liver...
Publication Info
- Year
- 1971
- Type
- article
- Volume
- 17
- Issue
- 12
- Pages
- 1154-1159
- Citations
- 155
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
155
OpenAlex
Cite This
Nathan Gochman,
Joan M Schmitz
(1971).
Automated Determination of Uric Acid, with Use of a Uricase—Peroxidase System.
Clinical Chemistry
, 17
(12)
, 1154-1159.
https://doi.org/10.1093/clinchem/17.12.1154
Identifiers
- DOI
- 10.1093/clinchem/17.12.1154