Abstract
Bovine lactoferricin is an antimicrobial, cationic peptide generated upon gastric pepsin cleavage of bovine lactoferrin. We investigated the bactericidal effects of native lactoferricin [Lfcin B(17-41)], a shortened derivative [Lfcin B(17-31)] and the all-D-amino acid counterpart of Lfcin B(17-31) against Escherichia coli and Staphylococcus aureus. The results revealed different activities for the peptides against Gram-positive and -negative bacteria. D-Lfcin B(17-31) was the most efficient peptide against E. coli. The same peptide showed improved activity against S. aureus, D-Lfcin B(17-31) showed a significant better efficacy when compared to the L-form, but not when compared to Lfcin B(17-41). There was no correlation between the bactericidal concentrations and the time needed to achieve maximum effect. This indicates the importance of structural differences between the peptides and/or bacteria and implies that the simple thesis of I antibacterial target is not valid for lactoferricin.
Keywords
LactoferrinEscherichia coliStaphylococcus aureusAntimicrobialMicrobiologyPeptideAntibacterial activityBiologyAntibacterial agentBacteriaAntibioticsBiochemistry
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Publication Info
- Year
- 2001
- Type
- article
- Volume
- 33
- Issue
- 7
- Pages
- 507-511
- Citations
- 47
- Access
- Closed
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Cite This
Hilde Ulvatne,
Lars H. Vorland
(2001).
Bactericidal Kinetics of 3 Lactoferricins Against Staphylococcus aureus and Escherichia coli.
Scandinavian Journal of Infectious Diseases
, 33
(7)
, 507-511.
https://doi.org/10.1080/00365540110026692
Identifiers
- DOI
- 10.1080/00365540110026692