Abstract

CD4(+)CD25(+) immunoregulatory T cells represent a unique lineage of thymic-derived cells that potently suppress both in vitro and in vivo effector T cell function. We analyzed CD4(+)CD25(+) and CD4(+)CD25(-) T cells by DNA microarray, identifying 29 genes differentially expressed in the resting subpopulations, and 77 that were differentially expressed following activation. Most of these genes were elevated in the CD4(+)CD25(+) population, suggesting a previously activated phenotype. Among these were a number of genes that antagonize signaling, including members of the SOCS family, which may contribute to their anergic phenotype. Multiple cell surface receptors also had increased expression in CD4(+)CD25(+) cells, including GITR, a member of the TNF receptor superfamily. Importantly, antibodies to GITR abrogated suppression, demonstrating a functional role for this receptor in regulating the CD4(+)CD25(+) T cell subset.

Keywords

IL-2 receptorBiologyCell biologyPhenotypeReceptorT cellEffectorPopulationMolecular biologyGeneImmunologyGeneticsImmune system

MeSH Terms

AnimalsAntibodiesMonoclonalAntigensCDBiomarkersCD4 AntigensCD4-Positive T-LymphocytesCell SeparationFemaleGene ExpressionGene Expression ProfilingGlucocorticoid-Induced TNFR-Related ProteinGlucocorticoidsIntegrin alpha ChainsInterleukin-2Lymphocyte ActivationMiceMiceInbred BALB CMiceInbred C57BLOligonucleotide Array Sequence AnalysisReceptorsCell SurfaceReceptorsInterleukin-2ReceptorsNerve Growth FactorReceptorsTumor Necrosis Factor

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Publication Info

Year
2002
Type
article
Volume
16
Issue
2
Pages
311-323
Citations
1393
Access
Closed

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Cite This

Rebecca S. McHugh, Matthew J. Whitters, Ciriaco A. Piccirillo et al. (2002). CD4+CD25+ Immunoregulatory T Cells. Immunity , 16 (2) , 311-323. https://doi.org/10.1016/s1074-7613(02)00280-7

Identifiers

DOI
10.1016/s1074-7613(02)00280-7
PMID
11869690

Data Quality

Data completeness: 86%