Abstract

Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and malignant human cells, we found RNA transcripts from many human genes in which the exons were arranged in a non-canonical order. Statistical estimates and biochemical assays provided strong evidence that a substantial fraction of the spliced transcripts from hundreds of genes are circular RNAs. Our results suggest that a non-canonical mode of RNA splicing, resulting in a circular RNA isoform, is a general feature of the gene expression program in human cells.

Keywords

ExonBiologyGeneGeneticsRNACircular RNAAlternative splicingRNA splicingIntronHuman genomeGene isoformNon-coding RNAPost-transcriptional modificationComputational biologyMolecular biologyGenome

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Year
2012
Type
article
Volume
7
Issue
2
Pages
e30733-e30733
Citations
2532
Access
Closed

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Julia Salzman, Charles Gawad, Peter Lincoln Wang et al. (2012). Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types. PLoS ONE , 7 (2) , e30733-e30733. https://doi.org/10.1371/journal.pone.0030733

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DOI
10.1371/journal.pone.0030733