Abstract

Complementary DNA clones encoding the human vitamin D receptor have been isolated from human intestine and T47D cell cDNA libraries. The nucleotide sequence of the 4605-base pair (bp) cDNA includes a noncoding leader sequence of 115 bp, a 1281-bp open reading frame, and 3209 bp of 3' noncoding sequence. Two polyadenylylation signals, AATAAA, are present 25 and 70 bp upstream of the poly(A) tail, respectively. RNA blot hybridization indicates a single mRNA species of approximately equal to 4600 bp. Transfection of the cloned sequences into COS-1 cells results in the production of a single receptor species indistinguishable from the native receptor. Sequence comparisons demonstrate that the vitamin D receptor belongs to the steroid-receptor gene family and is closest in size and sequence to another member of this family, the thyroid hormone receptor.

Keywords

Complementary DNABiologyMolecular biologycDNA libraryNucleic acid sequenceCoding regionGeneticsOpen reading frameGeneStop codonPeptide sequence

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Publication Info

Year
1988
Type
article
Volume
85
Issue
10
Pages
3294-3298
Citations
958
Access
Closed

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Andrew R. Baker, Donald P. McDonnell, Mark Hughes et al. (1988). Cloning and expression of full-length cDNA encoding human vitamin D receptor.. Proceedings of the National Academy of Sciences , 85 (10) , 3294-3298. https://doi.org/10.1073/pnas.85.10.3294

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DOI
10.1073/pnas.85.10.3294