Abstract

Abstract Functional characterization of a protein sequence is one of the most frequent problems in biology. This task is usually facilitated by accurate three‐dimensional (3‐D) structure of the studied protein. In the absence of an experimentally determined structure, comparative or homology modeling can sometimes provide a useful 3‐D model for a protein that is related to at least one known protein structure. Comparative modeling predicts the 3‐D structure of a given protein sequence (target) based primarily on its alignment to one or more proteins of known structure (templates). The prediction process consists of fold assignment, target‐template alignment, model building, and model evaluation. This unit describes how to calculate comparative models using the program MODELLER and discusses all four steps of comparative modeling, frequently observed errors, and some applications. Modeling lactate dehydrogenase from Trichomonas vaginalis (TvLDH) is described as an example. The download and installation of the MODELLER software is also described.

Keywords

MODELLERHomology modelingProtein structure predictionComputer scienceCASPThreading (protein sequence)Loop modelingProtein structureComputational biologySequence alignmentTemplateModel buildingPeptide sequenceProgramming languageBiologyGeneticsPhysicsBiochemistry

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Year
2006
Type
article
Volume
15
Issue
1
Pages
5.6.1-5.6.37
Citations
4583
Access
Closed

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Narayanan Eswar, Ben Webb, Marc A. Martí‐Renom et al. (2006). Comparative Protein Structure Modeling Using Modeller. Current Protocols in Bioinformatics , 15 (1) , 5.6.1-5.6.37. https://doi.org/10.1002/0471250953.bi0506s15

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DOI
10.1002/0471250953.bi0506s15