Abstract
The UK Biobank Genotyping Project demonstrated that the high throughput DNA extraction protocol described generates high quality DNA suitable for genotyping on the Affymetrix Axiom array. The correlation between DNA concentration derived from UV/Vis and PicoGreen® quantification methods suggests, in large-scale genetic studies involving two laboratories, it may be possible to remove the DNA quantification step in one laboratory without affecting downstream analyses. This would result in reductions in cost and time to complete the project, allowing generation of genetic data faster and cheaper.
Keywords
BiobankGenotypingSNP genotypingDNA extractionBiologyGeneticsComputational biologyGenotypeGenePolymerase chain reaction
Affiliated Institutions
Related Publications
UK Biobank: An Open Access Resource for Identifying the Causes of a Wide Range of Complex Diseases of Middle and Old Age
Cathie Sudlow and colleagues describe the UK Biobank, a large population-based prospective study, established to allow investigation of the genetic and non-genetic determinants ...
Publication Info
- Year
- 2017
- Type
- article
- Volume
- 18
- Issue
- 1
- Pages
- 26-26
- Citations
- 103
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
103
OpenAlex
Cite This
Samantha Welsh,
Tim Peakman,
Simon Sheard
et al.
(2017).
Comparison of DNA quantification methodology used in the DNA extraction protocol for the UK Biobank cohort.
BMC Genomics
, 18
(1)
, 26-26.
https://doi.org/10.1186/s12864-016-3391-x
Identifiers
- DOI
- 10.1186/s12864-016-3391-x