Abstract

We have compared the efficacies of three general primer pairs for the detection of human papillomavirus (HPV) DNA in formaldehyde-fixed paraffin-embedded carcinomas. The use of these primer pairs leads to underestimates of the HPV prevalence (GP5/6, 61.1%; CPI/IIG, 57.4%; MY09/11, 46.9%; combined, 72.8%). The efficacy of each primer pair seemed to be inversely correlated to the length of the amplimer produced. By using newly developed type-specific primer pairs (amplimer length, approximately 100 bp), an increase in HPV DNA detection (87.6%) was found.

Keywords

Primer (cosmetics)Human papillomavirusBiologyDNAPolymerase chain reactionMolecular biologyVirologyPapillomaviridaeCervical cancerGeneticsMedicineCancerGeneChemistryInternal medicine

MeSH Terms

Base SequenceDNA PrimersDNAViralFemaleHumansMolecular Sequence DataPapillomaviridaePolymerase Chain ReactionUterine Cervical Neoplasms

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Publication Info

Year
1996
Type
article
Volume
34
Issue
3
Pages
745-747
Citations
243
Access
Closed

External Links

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Cite This

Marc Baay, W G Quint, J Koudstaal et al. (1996). Comprehensive study of several general and type-specific primer pairs for detection of human papillomavirus DNA by PCR in paraffin-embedded cervical carcinomas. Journal of Clinical Microbiology , 34 (3) , 745-747. https://doi.org/10.1128/jcm.34.3.745-747.1996

Identifiers

DOI
10.1128/jcm.34.3.745-747.1996
PMID
8904451
PMCID
PMC228883

Data Quality

Data completeness: 86%