Crystal structure of Nsp15 endoribonuclease <scp>NendoU</scp> from <scp>SARS‐CoV</scp> ‐2

Youngchang Kim; R. Jedrzejczak; N. Maltseva; Mateusz Wilamowski; M. Endres; Adam Godzik; K. Michalska; A. Joachimiak

doi:10.1002/pro.3873

Abstract

Abstract Severe Acute Respiratory Syndrome coronavirus 2 (SARS‐CoV‐2) is rapidly spreading around the world. There is no existing vaccine or proven drug to prevent infections and stop virus proliferation. Although this virus is similar to human and animal SARS‐CoVs and Middle East Respiratory Syndrome coronavirus (MERS‐CoVs), the detailed information about SARS‐CoV‐2 proteins structures and functions is urgently needed to rapidly develop effective vaccines, antibodies, and antivirals. We applied high‐throughput protein production and structure determination pipeline at the Center for Structural Genomics of Infectious Diseases to produce SARS‐CoV‐2 proteins and structures. Here we report two high‐resolution crystal structures of endoribonuclease Nsp15/NendoU. We compare these structures with previously reported homologs from SARS and MERS coronaviruses.

Keywords

EndoribonucleaseVirologyCoronavirusMiddle East respiratory syndrome coronavirusSevere acute respiratory syndrome coronavirusVirusSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Middle East respiratory syndromeBiologyComputational biologyCoronavirus disease 2019 (COVID-19)MedicineGeneGeneticsInfectious disease (medical specialty)RNA

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Publication Info

Year: 2020
Type: article
Volume: 29
Issue: 7
Pages: 1596-1605
Citations: 360
Access: Closed

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APA Style

                            
                                
                                    Youngchang Kim, 
                                
                                    R. Jedrzejczak, 
                                
                                    N. Maltseva
                                
                                et al.
                            
                            (2020). 
                            Crystal structure of Nsp15 endoribonuclease <scp>NendoU</scp> from <scp>SARS‐CoV</scp> ‐2. 
                            Protein Science
                            , 29
                            (7)
                            , 1596-1605.
                            https://doi.org/10.1002/pro.3873
                        

Identifiers

DOI: 10.1002/pro.3873