Cyclic AMP-Induced Repair of Zebrafish Spinal Circuits

Dimple H. Bhatt; Stefanie Otto; Brett Depoister; Joseph R. Fetcho

doi:10.1126/science.1098439

Abstract

Neurons in the human central nervous system (CNS) are unable to regenerate, as a result of both an inhibitory environment and their inherent inability to regrow. In contrast, the CNS environment in fish is permissive for growth, yet some neurons still cannot regenerate. Fish thus offer an opportunity to study molecules that might surmount the intrinsic limitations they share with mammals, without the complication of an inhibitory environment. We show by in vivo imaging in zebrafish that post-injury application of cyclic adenosine monophosphate can transform severed CNS neurons into ones that regenerate and restore function, thus overcoming intrinsic limitations to regeneration in a vertebrate.

Keywords

ZebrafishNeuroscienceVertebrateRegeneration (biology)Inhibitory postsynaptic potentialCentral nervous systemBiologyFunction (biology)Spinal cordIn vivoNeuronal circuitsNervous systemFish <Actinopterygii>Biological neural networkCell biologyAnatomyBiochemistryGene

MeSH Terms

AnimalsAxonsBucladesineCalciumCyclic AMPElectroporationEscape ReactionFluorescent DyesInterneuronsMicroscopyConfocalNerve RegenerationNeuronsRegenerationRhodaminesSpinal CordSpinal Cord InjuriesZebrafish

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Publication Info

Year: 2004
Type: article
Volume: 305
Issue: 5681
Pages: 254-258
Citations: 183
Access: Closed

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APA Style

                            
                                
                                    Dimple H. Bhatt, 
                                
                                    Stefanie Otto, 
                                
                                    Brett Depoister
                                
                                et al.
                            
                            (2004). 
                            Cyclic AMP-Induced Repair of Zebrafish Spinal Circuits. 
                            Science
                            , 305
                            (5681)
                            , 254-258.
                            https://doi.org/10.1126/science.1098439
                        

Identifiers

DOI: 10.1126/science.1098439
PMID: 15247482

Data Quality

Data completeness: 81%