Abstract
Abstract Minimum inhibitory concentrations (MICs) are defined as the lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism after overnight incubation, and minimum bactericidal concentrations (MBCs) as the lowest concentration of antimicrobial that will prevent the growth of an organism after subculture on to antibiotic-free media. MICs are used by diagnostic laboratories mainly to confirm resistance, but most often as a research tool to determine the in vitro activity of new antimicrobials, and data from such studies have been used to determine MIC breakpoints. MBC determinations are undertaken less frequently and their major use has been reserved for isolates from the blood of patients with endocarditis. Standardized methods for determining MICs and MBCs are described in this paper. Like all standardized procedures, the method must be adhered to and may not be adapted by the user. The method gives information on the storage of standard antibiotic powder, preparation of stock antibiotic solutions, media, preparation of inocula, incubation conditions, and reading and interpretation of results. Tables giving expected MIC ranges for control NCTC and ATCC strains are also supplied.
Keywords
Minimum inhibitory concentrationAntimicrobialMinimum bactericidal concentrationSubculture (biology)MicrobiologyIncubationAntibioticsAntibiotic resistanceIncubation periodBiologyBiochemistry
MeSH Terms
Anti-Bacterial AgentsBacteriaMicrobial Sensitivity Tests
Affiliated Institutions
Related Publications
In Vitro Antibacterial Properties of Pexiganan, an Analog of Magainin
ABSTRACT Pexiganan, a 22-amino-acid antimicrobial peptide, is an analog of the magainin peptides isolated from the skin of the African clawed frog. Pexiganan exhibited in vitro ...
Comparison of three different in vitro methods of detecting synergy: time-kill, checkerboard, and E test
An in vitro method of detecting synergy which is simple to perform, accurate, and reproducible and has the potential for clinical extrapolation is desirable. Time-kill and check...
The Calgary Biofilm Device: New Technology for Rapid Determination of Antibiotic Susceptibilities of Bacterial Biofilms
ABSTRACT Determination of the MIC, based on the activities of antibiotics against planktonic bacteria, is the standard assay for antibiotic susceptibility testing. Adherent bact...
The mode of antimicrobial action of the essential oil of Melaleuca alternifolia (tea tree oil)
The essential oil of Melaleuca alternifolia (tea tree) exhibits broad-spectrum antimicrobial activity. Its mode of action against the Gram-negative bacterium Escherichia coli AG...
Mechanism of Action of<i>Melaleuca alternifolia</i>(Tea Tree) Oil on<i>Staphylococcus aureus</i>Determined by Time-Kill, Lysis, Leakage, and Salt Tolerance Assays and Electron Microscopy
ABSTRACT The essential oil of Melaleuca alternifolia (tea tree) has broad-spectrum antimicrobial activity. The mechanisms of action of tea tree oil and three of its components, ...
Publication Info
- Year
- 2001
- Type
- article
- Volume
- 48
- Issue
- suppl_1
- Pages
- 5-16
- Citations
- 3955
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
3955
OpenAlex
240
Influential
3048
CrossRef
Cite This
Jennifer M. Andrews
(2001).
Determination of minimum inhibitory concentrations.
Journal of Antimicrobial Chemotherapy
, 48
(suppl_1)
, 5-16.
https://doi.org/10.1093/jac/48.suppl_1.5
Identifiers
- DOI
- 10.1093/jac/48.suppl_1.5
- PMID
- 11420333