Abstract
Culture of human peripheral blood mononuclear cells (PBMC) with IL-2 stimulates synthesis of cytokines and generation of lymphokine-activated killer (LAK) activity. Both IL-4 and IL-10 [cytokine synthesis inhibitory factor (CSIF)] inhibit IL-2-induced synthesis of IFN-gamma and tumor necrosis factor (TNF)-alpha by human PBMC. However, unlike IL-4, IL-10 inhibits neither IL-2-induced proliferation of PBMC and fresh natural killer (NK) cells, nor IL-2-induced LAK activity. Moreover, IL-4 inhibits IL-2-induced IFN-gamma synthesis by purified fresh NK cells, while in contrast the inhibitory effect of IL-10 is mediated by CD14+ cells (monocytes/macrophages). IL-10 inhibits TNF-alpha synthesis by monocytes or monocytes plus NK cells, but not by NK cells alone. These results suggest that IL-4 and IL-10 act on NK cells via distinct pathways, and that IL-2-induced cytokine synthesis and LAK activity are regulated via different mechanisms.
Keywords
Lymphokine-activated killer cellLymphokineCytokineInterleukin 12Peripheral blood mononuclear cellTumor necrosis factor alphaNatural killer T cellChemistryInterleukin 2Interleukin 21ImmunologyBiologyMolecular biologyCytotoxic T cellT cellImmune systemIn vitroBiochemistry
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Publication Info
- Year
- 1992
- Type
- article
- Volume
- 4
- Issue
- 5
- Pages
- 563-569
- Citations
- 206
- Access
- Closed
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Cite This
Di-Hwel Hsu,
Kevin W. Moore,
Hergen Splts
(1992).
Differential effects of IL-4 and IL-10 on IL-2-induced IFN-γ synthesis and lymphokine-activated killer activity.
International Immunology
, 4
(5)
, 563-569.
https://doi.org/10.1093/intimm/4.5.563
Identifiers
- DOI
- 10.1093/intimm/4.5.563