Diffusion Dynamics of Glycine Receptors Revealed by Single-Quantum Dot Tracking

Abstract

Semiconductor quantum dots (QDs) are nanometer-sized fluorescent probes suitable for advanced biological imaging. We used QDs to track individual glycine receptors (GlyRs) and analyze their lateral dynamics in the neuronal membrane of living cells for periods ranging from milliseconds to minutes. We characterized multiple diffusion domains in relation to the synaptic, perisynaptic, or extrasynaptic GlyR localization. The entry of GlyRs into the synapse by diffusion was observed and further confirmed by electron microscopy imaging of QD-tagged receptors.

Keywords

Glycine receptorQuantum dotGlycineBiophysicsSynapseReceptorDiffusionLateral diffusionFluorescenceNanotechnologyChemistryMembraneMaterials scienceNeurosciencePhysicsBiologyOpticsBiochemistry

MeSH Terms

AnimalsCell MembraneCellsCulturedDendritesDiffusionFluorescenceFluorescent DyesMicroscopyElectronNanotechnologyNeuritesNeuronsPyridinium CompoundsQuaternary Ammonium CompoundsRatsRatsSprague-DawleyReceptorsGlycineSemiconductorsSpinal CordSynapses

Affiliated Institutions

Related Publications

GABA and glycine as neurotransmitters: a brief history

Norman G. Bowery , Trevor G. Smart

γ ‐Aminobutyric acid (GABA) emerged as a potentially important brain chemical just over 50 years ago, but its significance as a neurotransmitter was not fully realized until ove...

2006 British Journal of Pharmacology 320 citations

Behavioral and autonomic correlates of the tactile evoked allodynia produced by spinal glycine inhibition: effects of modulatory receptor systems and excitatory amino acid antagonists

Tony L. Yaksh

Intrathecal administration of glycine (strychnine) or GABA (bicuculline) but not opioid (naloxone), adrenergic (phentolamine) or serotonin (methysergide) receptor antagonists re...

1989 Pain 673 citations

Single molecule studies of quantum dot conjugates in a submicrometer fluidic channel

Samuel M. Stavis , Joshua B. Edel , Kevan T. Samiee +1 more

A microfluidic and optical system was created for the detection and analysis of single molecules in solution. Fluidic channels with submicrometer dimensions were used to isolate...

2005 Lab on a Chip 88 citations

Near-infrared fluorescent type II quantum dots for sentinel lymph node mapping

Sungjee Kim , Yong Taik Lim , Edward G. Soltesz +10 more

2003 Nature Biotechnology 2067 citations

Visible light emission due to quantum size effects in highly porous crystalline silicon

A.G. Cullis , Leigh Canham

1991 Nature 1404 citations

Publication Info

Year: 2003
Type: article
Volume: 302
Issue: 5644
Pages: 442-445
Citations: 1453
Access: Closed

External Links

Download PDF (Free) View on DOI.org PubMed Semantic Scholar

Social Impact

Altmetric

Diffusion Dynamics of Glycine Receptors Revealed by Single-Quantum Dot Tracking

PlumX Metrics

Social media, news, blog, policy document mentions

Citation Metrics

1453

OpenAlex

Influential

1255

CrossRef

Cite This

APA Style

                            
                                
                                    Maxime Dahan, 
                                
                                    Sabine Lévi, 
                                
                                    Camilla Luccardini
                                
                                et al.
                            
                            (2003). 
                            Diffusion Dynamics of Glycine Receptors Revealed by Single-Quantum Dot Tracking. 
                            Science
                            , 302
                            (5644)
                            , 442-445.
                            https://doi.org/10.1126/science.1088525
                        

Identifiers

DOI: 10.1126/science.1088525
PMID: 14564008

Data Quality

Data completeness: 81%