Direct database searching with MALDI‐PSD spectra of peptides

Abstract

Abstract The analysis of matrix‐assisted laser desorption ionization post‐source decay (MALDI‐PSD) mass spectra of peptides by using the cross‐correlation method for database searching is illustrated. MALDI‐PSD mass spectra are shown to contain sufficient fragmentation information to uniquely identify the correct amino acid sequence from large protein databases (∼160000 entries). A search employing the MALDI‐PSD mass spectrum of a phosphorylated peptide that correctly identifies the amino acid sequence and the site of phosphorylation is also illustrated.

Keywords

ChemistryMatrix-assisted laser desorption/ionizationMass spectrumFragmentation (computing)PeptideMass spectrometryPeptide sequenceSpectral lineDatabase search engineDatabaseIonizationAmino acidSequence (biology)ChromatographyDesorptionBiochemistryPhysical chemistryOrganic chemistryInformation retrievalIonSearch enginePhysics

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Publication Info

Year: 1995
Type: article
Volume: 9
Issue: 15
Pages: 1546-1551
Citations: 84
Access: Closed

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APA Style

                            
                                
                                    Patrick R. Griffin, 
                                
                                    Michael J. MacCoss, 
                                
                                    Richard Blevins
                                
                                et al.
                            
                            (1995). 
                            Direct database searching with MALDI‐PSD spectra of peptides. 
                            Rapid Communications in Mass Spectrometry
                            , 9
                            (15)
                            , 1546-1551.
                            https://doi.org/10.1002/rcm.1290091515
                        

Identifiers

DOI: 10.1002/rcm.1290091515