Abstract

Abstract The in vitro synthesis of cellulose via a nonbiosynthetic path has been achieved for the first time by condensation of β‐D‐cellobiosyl fluoride as substrate for cellulase in a mixed solvent of acetonitrile/acetate buffer (pH 5,5:1) ( enzymatic polymerization ). Under reaction condition of a higher substrate concentration or higher acetonitrile concentration, cellooligosaccharides were produced predominantly. The present method of using a glycosyl fluoride as substrate for a hydrolytic enzyme in an organic/water mixed solvent has successfully been applied to synthesis of maltooligosaccharides and unnatural oligosaccharides.

Keywords

CellulaseChemistryCelluloseAcetonitrileSubstrate (aquarium)HydrolysisPolymerizationGlycosylSolventCatalysisOrganic chemistryFluoridePolymer chemistryCellulose acetatePolymerInorganic chemistry

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Publication Info

Year
1992
Type
article
Volume
54-55
Issue
1
Pages
509-518
Citations
46
Access
Closed

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Shiro Kobayashi, Keita Kashiwa, Junji Shimada et al. (1992). Enzymatic polymerization: The first in vitro synthesis of cellulose via nonbiosynthetic path catalyzed by cellulase. Makromolekulare Chemie Macromolecular Symposia , 54-55 (1) , 509-518. https://doi.org/10.1002/masy.19920540138

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DOI
10.1002/masy.19920540138