Abstract

16S rRNA gene sequences have been widely used for the identification of prokaryotes. However, the flood of sequences of non-type strains and the lack of a peer-reviewed database for 16S rRNA gene sequences of type strains have made routine identification of isolates difficult and labour-intensive. In the present study, we generated a database containing 16S rRNA gene sequences of all prokaryotic type strains. In addition, a web-based tool, named EzTaxon, for analysis of 16S rRNA gene sequences was constructed to achieve identification of isolates based on pairwise nucleotide similarity values and phylogenetic inference methods. The system developed provides users with a similarity-based search, multiple sequence alignment and various phylogenetic analyses. All of these functions together with the 16S rRNA gene sequence database of type strains can be successfully used for automated and reliable identification of prokaryotic isolates. The EzTaxon server is freely accessible over the Internet at http://www.eztaxon.org/

Keywords

Biology16S ribosomal RNAPhylogenetic treeGeneRibosomal RNAGeneticsComputational biologyIdentification (biology)Sequence analysis

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Year
2007
Type
article
Volume
57
Issue
10
Pages
2259-2261
Citations
2103
Access
Closed

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Jongsik Chun, Jae‐Hak Lee, Yoonyoung Jung et al. (2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY , 57 (10) , 2259-2261. https://doi.org/10.1099/ijs.0.64915-0

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DOI
10.1099/ijs.0.64915-0