Far-red fluorescent tag for protein labelling

Abstract

Practical applications of green fluorescent protein ('GFP')-like fluorescent proteins (FPs) from species of the class Anthozoa (sea anemones, corals and sea pens) are strongly restricted owing to their oligomeric nature. Here we suggest a strategy to overcome this problem by the use of two covalently linked identical red FPs as non-oligomerizing fusion tags. We have applied this approach to the dimeric far-red fluorescent protein HcRed1 and have demonstrated superiority of the tandem tag in the in vivo labelling of fine cytoskeletal structures and tiny nucleoli. In addition, a possibility of effective fluorescence resonance energy transfer ('FRET') between enhanced yellow FP mutant ('EYFP') and tandem HcRed1 was demonstrated in a protease assay.

Keywords

Förster resonance energy transferFluorescenceGreen fluorescent proteinLabellingProtein tagFusion proteinBiologyBimolecular fluorescence complementationBiophysicsFluorescent proteinBiochemistryChemistryRecombinant DNAGene

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Publication Info

Year: 2002
Type: article
Volume: 368
Issue: 1
Pages: 17-21
Citations: 81
Access: Closed

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APA Style

                            
                                
                                    Arkady F. Fradkov, 
                                
                                    Vladislav V. Verkhusha, 
                                
                                    Dmitriy B. Staroverov
                                
                                et al.
                            
                            (2002). 
                            Far-red fluorescent tag for protein labelling. 
                            Biochemical Journal
                            , 368
                            (1)
                            , 17-21.
                            https://doi.org/10.1042/bj20021191
                        

Identifiers

DOI: 10.1042/bj20021191