Abstract
Escherichia coli is one of the best elucidated organisms. However, about 40% of E. coli genes have not been assigned to their function yet. We analyzed 1440 single gene knock-out mutants using the GN2-MicroPlate, which permits assay of 95 carbon-source utilizations simultaneously. In the knock-out library there are 1044 of so called y-genes with no apparent function. The raw dataset was analyzed and genes were interrelated by the clustering method of the GeneSpring software. In the resulted dendrogram of genes, a group of genes with known and related function tended to be assembled into a cluster. Our clustering method would be useful for functional assignment of so called y-genes with no apparent function, since the resulted dendrogram could connect y-genes to phenotype and function of well-studied genes.
Keywords
DendrogramGeneEscherichia coliGeneticsPhenotypeBiologyFunction (biology)DNA microarrayGene clusterComputational biologyMutantFunctional analysisGene expression
MeSH Terms
Escherichia coliGene DeletionGene Expression ProfilingGene Expression RegulationBacterialGenesBacterialOligonucleotide Array Sequence AnalysisPhenotypeSoftware
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Publication Info
- Year
- 2005
- Type
- article
- Volume
- 7
- Issue
- 4
- Pages
- 318-327
- Citations
- 47
- Access
- Closed
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Cite This
Mikito Ito,
Tomoya Baba,
Hirotada Mori
et al.
(2005).
Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays.
Metabolic Engineering
, 7
(4)
, 318-327.
https://doi.org/10.1016/j.ymben.2005.06.004
Identifiers
- DOI
- 10.1016/j.ymben.2005.06.004
- PMID
- 16095938