Genome assembly using Nanopore-guided long and error-free DNA reads

Abstract

We described NaS tool, a hybrid approach allowing the sequencing of microbial genomes using the MinION® device. Our method, based ideally on 20x and 50x of NaS and Illumina reads respectively, provides an efficient and cost-effective way of sequencing microbial or small eukaryotic genomes in a very short time even in small facilities. Moreover, we demonstrated that although the Oxford Nanopore technology is a relatively new sequencing technology, currently with a high error rate, it is already useful in the generation of high-quality genome assemblies.

Keywords

MinionNanopore sequencingHybrid genome assemblyContigSequence assemblyGenomeComputational biologyNanoporeBiologyDNA sequencingComputer scienceDNA sequencerReference genomeGeneticsDNAGeneNanotechnology

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Publication Info

Year: 2015
Type: article
Volume: 16
Issue: 1
Pages: 327-327
Citations: 191
Access: Closed

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APA Style

                            
                                
                                    Mohammed‐Amin Madoui, 
                                
                                    Stéfan Engelen, 
                                
                                    Corinne Cruaud
                                
                                et al.
                            
                            (2015). 
                            Genome assembly using Nanopore-guided long and error-free DNA reads. 
                            BMC Genomics
                            , 16
                            (1)
                            , 327-327.
                            https://doi.org/10.1186/s12864-015-1519-z
                        

Identifiers

DOI: 10.1186/s12864-015-1519-z