Abstract
A physical map of the genome of Clostridium perfringens, an important human pathogen, has been established by pulsed-field gel electrophoresis. Recognition sites for six rare-cutting endonucleases were situated on a single circular chromosome of approximately 3.6 million base pairs thus defining 50 arbitrary genetic intervals of between 10 and 250 kilobase pairs. This considerably facilitated the chromosomal localization of some 24 genes and loci for which probes were available and allowed the construction of the genome map of a clostridial species.
Keywords
Clostridium perfringensGenomeBiologyGeneticsChromosomeGenePathogenClostridiumComputational biologyGenome sizeBacteria
Affiliated Institutions
Related Publications
Lateral gene transfer of an ABC transporter complex between major constituents of the human gut microbiome
Several links have been established between the human gut microbiome and conditions such as obesity and inflammatory bowel syndrome. This highlights the importance of understand...
Direct sequencing of the human microbiome readily reveals community differences
Culture-independent studies of human microbiota by direct genomic sequencing reveal quite distinct differences among communities, indicating that improved sequencing capacity ca...
Evolution of Symbiotic Bacteria in the Distal Human Intestine
The adult human intestine contains trillions of bacteria, representing hundreds of species and thousands of subspecies. Little is known about the selective pressures that have s...
Publication Info
- Year
- 1989
- Type
- article
- Volume
- 86
- Issue
- 17
- Pages
- 6676-6680
- Citations
- 163
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
163
OpenAlex
Cite This
Bruno Canard,
S T Cole
(1989).
Genome organization of the anaerobic pathogen Clostridium perfringens..
Proceedings of the National Academy of Sciences
, 86
(17)
, 6676-6680.
https://doi.org/10.1073/pnas.86.17.6676
Identifiers
- DOI
- 10.1073/pnas.86.17.6676