Abstract

Abstract Current methods for system‐wide gene expression analysis detect changes in mRNA abundance, but neglect regulation at the level of translation. Pulse labeling with stable isotopes has been used to measure protein turnover rates, but this does not directly provide information about translation rates. Here, we developed pulsed stable isotope labeling by amino acids in cell culture (pSILAC) with two heavy isotope labels to directly quantify protein translation on a proteome‐wide scale. We applied the method to cellular iron homeostasis as a model system and demonstrate that it can confidently identify proteins that are translationally regulated by iron availability.

Keywords

Stable isotope labeling by amino acids in cell cultureTranslation (biology)Protein biosynthesisProteomeProtein turnoverComputational biologyGene expressionCell biologyIsotopeProteomicsMessenger RNAChemistryBiologyBiochemistryGene

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Publication Info

Year
2008
Type
article
Volume
9
Issue
1
Pages
205-209
Citations
355
Access
Closed

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Björn Schwanhäußer, Manfred Gossen, Gunnar Dittmar et al. (2008). Global analysis of cellular protein translation by pulsed SILAC. PROTEOMICS , 9 (1) , 205-209. https://doi.org/10.1002/pmic.200800275

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DOI
10.1002/pmic.200800275