Heparin Affinity: Purification of a Tumor-Derived Capillary Endothelial Cell Growth Factor

Abstract

A tumor-derived growth factor that stimulates the proliferation of capillary endothelial cells has a very strong affinity for heparin. This heparin affinity makes it possible to purify the growth factor to a single-band preparation in a rapid two-step procedure. The purified growth factor is a cationic polypeptide, has a molecular weight of about 18,000, and stimulates capillary endothelial cell proliferation at a concentration of about 1 nanogram per milliliter.

Keywords

HeparinGrowth factorChemistryCell growthCapillary actionAffinity chromatographyChromatographyEndothelial stem cellCationic polymerizationBiochemistryBiophysicsBiologyIn vitroReceptorMaterials science

MeSH Terms

Angiogenesis Inducing AgentsAnimalsCapillariesCell DivisionCell LineChick EmbryoChondrosarcomaChromatographyAffinityDNAEndotheliumGrowth SubstancesHeparinMiceNeovascularizationPathologic

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Publication Info

Year: 1984
Type: article
Volume: 223
Issue: 4642
Pages: 1296-1299
Citations: 934
Access: Closed

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APA Style

                            
                                
                                    Yuen Shing, 
                                
                                    Judah Folkman, 
                                
                                    Robert Sullivan
                                
                                et al.
                            
                            (1984). 
                            Heparin Affinity: Purification of a Tumor-Derived Capillary Endothelial Cell Growth Factor. 
                            Science
                            , 223
                            (4642)
                            , 1296-1299.
                            https://doi.org/10.1126/science.6199844
                        

Identifiers

DOI: 10.1126/science.6199844
PMID: 6199844

Data Quality

Data completeness: 81%