Abstract
Endothelial cell growth factor (ECGF), an anionic polypeptide mitogen, binds to immobilized heparin. The interaction between the acidic polypeptide and the anionic carbohydrate suggests a mechanism that is independent of ion exchange. Monoclonal antibodies to purified bovine ECGF inhibited the biological activity of ECGF in crude preparations of bovine brain. These data indicate that ECGF is the principal mitogen for endothelial cells from bovine brain, that heparin affinity chromatography may be used to purify and concentrate ECGF, and that the affinity of ECGF for heparin may have structural and perhaps biological significance.
Keywords
HeparinAffinity chromatographyEndothelial stem cellBiochemistryMonoclonal antibodyCellBiological activityGrowth factorChemistryAntibodyBiologyCell biologyEnzymeImmunologyReceptorIn vitro
MeSH Terms
AnimalsAntibodiesMonoclonalBrain ChemistryCattleCell DivisionChromatographyAffinityEndothelial Growth FactorsEndotheliumGrowth SubstancesHeparinIon Exchange
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Publication Info
- Year
- 1984
- Type
- article
- Volume
- 225
- Issue
- 4665
- Pages
- 932-935
- Citations
- 387
- Access
- Closed
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Cite This
Thomas Maciag,
Tevie Mehlman,
Robert Friesel
et al.
(1984).
Heparin Binds Endothelial Cell Growth Factor, the Principal Endothelial Cell Mitogen in Bovine Brain.
Science
, 225
(4665)
, 932-935.
https://doi.org/10.1126/science.6382607
Identifiers
- DOI
- 10.1126/science.6382607
- PMID
- 6382607