Abstract

We are developing a laser based technique for the rapid sequencing of large fragments (approximately 40 kb) of DNA based upon the detection of single, fluorescently tagged nucleotides cleaved from a single DNA fragment. We have demonstrated significant progress on several of the important steps of this technique. The projected rate of sequencing is several hundred bases per second which is orders of magnitude faster than existing methods. Once developed, this technology could be utilized by investigators for rapid sequencing of genetic material from virtually any source.

Keywords

Single molecule real time sequencingDNA sequencingDNADNA nanoball sequencingSequencing by hybridizationComputational biologyFluorescenceNucleotideSequencing by ligationFragment (logic)BiologyDNA sequencerChemistryMolecular biologyGeneticsGenomic libraryGeneComputer scienceBase sequenceAlgorithmPhysics

MeSH Terms

Base SequenceDNAExonucleasesFluorescent DyesFluorometryLasersMethods

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Publication Info

Year
1989
Type
article
Volume
7
Issue
2
Pages
301-309
Citations
155
Access
Closed

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Cite This

James H. Jett, Richard A. Keller, John C. Martin et al. (1989). High-Speed DNA Sequencing: An Approach Based Upon Fluorescence Detection of Single Molecules. Journal of Biomolecular Structure and Dynamics , 7 (2) , 301-309. https://doi.org/10.1080/07391102.1989.10507773

Identifiers

DOI
10.1080/07391102.1989.10507773
PMID
2557861

Data Quality

Data completeness: 90%