Identification of a Peptide Recognized by Five Melanoma-Specific Human Cytotoxic T Cell Lines

Abstract

Of several thousand peptides presented by the major histocompatibility molecule HLA-A2.1, at least nine are recognized by melanoma-specific cytotoxic T lymphocytes (CTLs). Tandem mass spectrometry was used to identify and to sequence one of these peptide epitopes. Melanoma-specific CTLs had an exceptionally high affinity for this nine-residue peptide, which reconstituted an epitope for CTL lines from each of five different melanoma patients tested. Recognition by multiple CTL lines suggests that this may be a promising candidate for use in peptide-based melanoma vaccines.

Keywords

CTL*Cytotoxic T cellEpitopePeptideMelanomaMajor histocompatibility complexHuman leukocyte antigenBiologyPeptide sequenceImmunologyCancer researchMolecular biologyChemistryAntigenBiochemistryIn vitroGene

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Publication Info

Year: 1994
Type: article
Volume: 264
Issue: 5159
Pages: 716-719
Citations: 829
Access: Closed

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APA Style

                            
                                
                                    Andrea L. Cox, 
                                
                                    Jonathan Skipper, 
                                
                                    Ye Chen
                                
                                et al.
                            
                            (1994). 
                            Identification of a Peptide Recognized by Five Melanoma-Specific Human Cytotoxic T Cell Lines. 
                            Science
                            , 264
                            (5159)
                            , 716-719.
                            https://doi.org/10.1126/science.7513441
                        

Identifiers

DOI: 10.1126/science.7513441