miR-15 and miR-16 induce apoptosis by targeting BCL2

Amelia Cimmino; George A. Calin; Muller Fabbri; Marilena V. Iorio; Manuela Ferracin; Masayoshi Shimizu; Sylwia E. Wojcik; Rami I. Aqeilan; Simona Zupo; Mariella Dono; Laura Z. Rassenti; Hansjüerg Alder; Stefano Volinia; Chang‐Gong Liu; Thomas J. Kipps; Massimo Negrini; Carlo M. Croce

doi:10.1073/pnas.0506654102

Abstract

Chronic lymphocytic leukemia (CLL) is the most common human leukemia and is characterized by predominantly nondividing malignant B cells overexpressing the antiapoptotic B cell lymphoma 2 (Bcl2) protein. miR-15a and miR-16-1 are deleted or down-regulated in the majority of CLLs. Here, we demonstrate that miR-15a and miR-16-1 expression is inversely correlated to Bcl2 expression in CLL and that both microRNAs negatively regulate Bcl2 at a posttranscriptional level. BCL2 repression by these microRNAs induces apoptopsis in a leukemic cell line model. Therefore, miR-15 and miR-16 are natural antisense Bcl2 interactors that could be used for therapy of Bcl2-overexpressing tumors.

Keywords

microRNAChronic lymphocytic leukemiaCancer researchLeukemiaBiologyApoptosisPsychological repressionCell cultureLymphomaMolecular biologyGeneGene expressionImmunologyGenetics

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Publication Info

Year: 2005
Type: article
Volume: 102
Issue: 39
Pages: 13944-13949
Citations: 3474
Access: Closed

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APA Style

                            
                                
                                    Amelia Cimmino, 
                                
                                    George A. Calin, 
                                
                                    Muller Fabbri
                                
                                et al.
                            
                            (2005). 
                            <i>miR-15</i> and <i>miR-16</i> induce apoptosis by targeting BCL2. 
                            Proceedings of the National Academy of Sciences
                            , 102
                            (39)
                            , 13944-13949.
                            https://doi.org/10.1073/pnas.0506654102
                        

Identifiers

DOI: 10.1073/pnas.0506654102

<i>miR-15</i> and <i>miR-16</i> induce apoptosis by targeting BCL2