Abstract
Abstract A rapid method is described for extraction and cleanup of raw and processed milk for determination of anatoxins M1 and M2 by using a C18 Sep-Pak/silica gel cleanup column combination. Anatoxins are separated by normal phase liquid chromatography and their concentrations are determined by fluorescence detection in a silica gel-packed flow cell. Recoveries ranged from 99 to 103% with coefficients of variation less than 2% for Mi levels of 0.117-1.17 ng/mL added to raw milk. Similar recoveries were obtained for M2. The coefficient of variation for analysis of 5 subsamples of naturally contaminated milk was Jess than 1%. Agreement with the official method is satisfactory. Each sample requires less than 25 mL solvent and 10 min actual handling time. Sample chromatograms show no interferences in the M1-M2 elution region and no late-eluting peaks, which permits spacing injections at 13-20 min intervals. Anatoxin levels as low as 0.03 ppb may be determined by this procedure. Extracts have also been analyzed by thin layer chromatography.
Keywords
ChromatographyElutionAflatoxinChemistryCoefficient of variationRaw milkSilica gelSolid phase extractionHigh-performance liquid chromatographyExtraction (chemistry)ContaminationCartridgeSample preparationSolventMaterials science
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Publication Info
- Year
- 1984
- Type
- article
- Volume
- 67
- Issue
- 6
- Pages
- 1111-1114
- Citations
- 12
- Access
- Closed
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Cite This
Jane Ferguson-Foos,
Joseph D. Warren
(1984).
Improved Cleanup for Liquid Chromatographic Analysis and Fluorescence Detection of Aflatoxins M1 and M2 in Fluid Milk Products.
Journal of AOAC INTERNATIONAL
, 67
(6)
, 1111-1114.
https://doi.org/10.1093/jaoac/67.6.1111
Identifiers
- DOI
- 10.1093/jaoac/67.6.1111