Abstract

Carbachol stimulation of rat parotid gland fragments prelabelled with myo-[3H]-inositol results in a large accumulation after 15 min of [3H]inositol trisphosphate. Only some of this is the D-1,4,5 isomer which would be expected to be derived from the known phosphatidylinositol bisphosphate. The predominant inositol trisphosphate is not susceptible to hydrolysis by human erythrocyte membranes. It yields altritol after periodate treatment followed by reduction and dephosphorylation, and, from partial dephosphorylation experiments, does not have a phosphate in the 2 position; the most likely structure of this inositol trisphosphate is therefore (D/L)-myo-inositol 1,3,4-trisphosphate. The possible origin and significance of this compound are discussed.

Keywords

DephosphorylationInositolPhosphatidylinositolCarbacholInositol trisphosphateInositol phosphateParotid glandStimulationChemistryPhosphorylationBiochemistryEndocrinologyInternal medicineBiologyReceptorPhosphataseMedicinePathology

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Year
1984
Type
article
Volume
223
Issue
1
Pages
237-243
Citations
297
Access
Closed

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R.F. Irvine, Amber Letcher, D. J. Lander et al. (1984). Inositol trisphosphates in carbachol-stimulated rat parotid glands. Biochemical Journal , 223 (1) , 237-243. https://doi.org/10.1042/bj2230237

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DOI
10.1042/bj2230237