Inside-Out Red Cell Membrane Vesicles: Preparation and Purification

Abstract

Plasma membranes purified from human red cells were converted into small vesicles by disruption in alkaline buffer of low ionic strength. Most of these vesicles were inside-out. The presence of divalent cations prevented this inversion. The inside-out vesicles were separated from right-side-out vesicles by centrifugation to equilibrium in dextran density gradients.

Keywords

VesicleDivalentMembraneChemistryBiophysicsChromatographyIonic strengthDextranBiochemistryBiologyOrganic chemistry

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Publication Info

Year: 1970
Type: article
Volume: 168
Issue: 3928
Pages: 255-257
Citations: 409
Access: Closed

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APA Style

                            
                                
                                    Theodore L. Steck, 
                                
                                    Ronald S. Weinstein, 
                                
                                    John H. Straus
                                
                                et al.
                            
                            (1970). 
                            Inside-Out Red Cell Membrane Vesicles: Preparation and Purification. 
                            Science
                            , 168
                            (3928)
                            , 255-257.
                            https://doi.org/10.1126/science.168.3928.255
                        

Identifiers

DOI: 10.1126/science.168.3928.255