Abstract
The properties of subcellular fractions of rat liver in catalyzing the decomposition of linoleic acid hydroperoxide have been compared with those of transition salts, heme compounds, and nucleophiles. The properties compared included the range of products produced, the pH dependence of the reaction, and the effects of metal-complexing agents, inhibitors, and hydrogen donors. It was concluded that the decomposition of the hydroperoxide in the liver cell was due principally to reaction with the intracellular nucleophile glutathione by a mechanism catalyzed by the enzyme glutathione peroxidase. In the absence of glutathione, however, both the mitochondrial and microsomal fractions decomposed the hydroperoxide presumably by a radical mechanism probably involving the cytochromes.
Keywords
ChemistryHydrogen peroxideDecompositionGlutathioneNucleophileIntracellularLipid peroxideMicrosomePeroxidaseBiochemistryPeroxideAntioxidantEnzymeCatalysisOrganic chemistryLipid peroxidation
Related Publications
Light-dependent Reduction of Oxidized Glutathione by Ruptured Chloroplasts
Crude extracts of pea shoots (Pisum sativum) catalyzed oxidized glutathione (GSSG)-dependent oxidation of NADPH which was attributed to NADPH-specific glutathione reductase. The...
Properties and Regulation of Glutathione Peroxidase
Abstract GSH peroxidase has been purified approximately 2500-fold from pig's blood. With cumene hydroperoxide as substrate, kinetic analysis on the purified enzyme gave nonlinea...
The Metabolic Fate of Mitochondrial Hydrogen Peroxide
Mitochondrial H 2 O 2 formation is not in equilibrium with defence mechanisms that counteract an accumulation of H 2 , in rat‐heart cell. A model for the accumulation kinetics i...
Nucleophilic Degradation of Fenitrothion Insecticide and Performance of Nucleophiles: A Computational Study
Ab initio and density functional theory (DFT) calculations have been performed to understand the destruction chemistry of an important organophosphorus insecticide O,O-dimethyl ...
Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts
Abstract Intact spinach chloroplasts scavenge hydrogen peroxide with a peroxidase that uses a photoreductant as the electron donor, but the activity of ruptured chloroplasts is ...
Publication Info
- Year
- 1969
- Type
- article
- Volume
- 47
- Issue
- 5
- Pages
- 493-499
- Citations
- 94
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
94
OpenAlex
Cite This
Peter J. O'Brien,
Christopher B. Little
(1969).
Intracellular mechanisms for the decomposition of a lipid peroxide. II. Decomposition of a lipid peroxide by subcellular fractions.
Canadian Journal of Biochemistry
, 47
(5)
, 493-499.
https://doi.org/10.1139/o69-077
Identifiers
- DOI
- 10.1139/o69-077