Abstract
Circular non-polyadenylated RNA molecules have been identified as stable transcription products of the human ETS-1 and mouse Sry genes. RNA circularization has been proposed to require two steps. The first step utilizes intramolecular base pairing to produce a transient stem-loop structure. The second step involves splicing a downstream donor splice site (DSS) to a now closely appositioned upstream acceptor splice site (ASS) within the loop. We demonstrate that the presence of long inverted repeats (IR) flanking the mouse Sry gene leads to the formation of the Sry circular transcript in cultured cells. Circularization requires the presence of both IR. As few as 400 complementary nt are necessary for this process. The presence of the IR does not significantly stimulate intermolecular annealing and trans-splicing in vivo.
Keywords
BiologyTestis determining factorRNA splicingPolyadenylationRNAStem-loopMolecular biologyGeneTranscription (linguistics)GeneticsAlternative splicingMessenger RNACell biologyY chromosome
Affiliated Institutions
Related Publications
Exon circularization in mammalian nuclear extracts.
Correct ligation of exons in pre-mRNA splicing requires splice site juxtaposition (splice site pairing), usually involving a 5' splice site and a downstream 3' splice site. Spli...
Mis-splicing yields circular RNA molecules.
We previously identified novel human ets-1 transcripts in which the normal order of exons is inverted, and demonstrated that although the order of exons is different than in the...
A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial–mesenchymal transition
Expression of Snail1 in epithelial cells triggers an epithelial–mesenchymal transition (EMT). Here, we demonstrate that the synthesis of Zeb2, a transcriptional repressor of E-c...
<i>U2AF1</i> mutations alter splice site recognition in hematological malignancies
Whole-exome sequencing studies have identified common mutations affecting genes encoding components of the RNA splicing machinery in hematological malignancies. Here, we sought ...
Conserved, developmentally regulated mechanism couples chromosomal looping and heterochromatin barrier activity at the homeobox gene A locus
Establishment and segregation of distinct chromatin domains are essential for proper genome function. The insulator protein CCCTC-binding factor (CTCF) is involved in creating b...
Publication Info
- Year
- 1995
- Type
- article
- Volume
- 167
- Issue
- 1-2
- Pages
- 245-248
- Citations
- 156
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
156
OpenAlex
Cite This
Robert Dubin,
Manija A. Kazmi,
Harry Ostrer
(1995).
Inverted repeats are necessary for circularization of the mouse testis Sry transcript.
Gene
, 167
(1-2)
, 245-248.
https://doi.org/10.1016/0378-1119(95)00639-7
Identifiers
- DOI
- 10.1016/0378-1119(95)00639-7