Abstract

The physiological action of glutamate is terminated by diffusional processes with its subsequent removal by high-affinity transport systems localized to glial and/or neuronal elements. Several cDNAs encoding glutamate transporters have been isolated from mammalian tissues. Here, we screened a cDNA library derived from mouse cerebellum and isolated the two glutamate transporters, termed MGLT1 and MEAAC1. The MGLT1 and MEAAC1 cDNAs encode proteins of 572 and 523 amino acids, respectively. MGLT1 has 93.9% amino acid sequence identity with the rat GLT1 and MEAAC1 has 89.3% identity with the rabbit EAAC1. MEAAC1 mRNA was expressed in brain, lung, kidney and skeletal muscle, whereas expression of MGLT1 was restricted to brain.

Keywords

Glutamate receptorComplementary DNATransporterAmino acidCloning (programming)Excitatory amino-acid transporterBiologycDNA libraryMolecular cloningMessenger RNACerebellumBiochemistryMolecular biologyGeneCell biologyNeuroscienceReceptor

MeSH Terms

ATP-Binding Cassette TransportersAmino Acid SequenceAmino Acid Transport System X-AGAnimalsBase SequenceCloningMolecularDNA PrimersDNAComplementaryGene ExpressionMiceMolecular Sequence DataRNAMessengerSequence AlignmentSequence HomologyAmino Acid

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Publication Info

Year
1995
Type
article
Volume
1244
Issue
1
Pages
233-237
Citations
40
Access
Closed

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Cite This

Yuri Mukainaka, Kohichi Tanaka, Tatsuya Hagiwara et al. (1995). Molecular cloning of two glutamate transporter subtypes from mouse brain. Biochimica et Biophysica Acta (BBA) - General Subjects , 1244 (1) , 233-237. https://doi.org/10.1016/0304-4165(95)00062-g

Identifiers

DOI
10.1016/0304-4165(95)00062-g
PMID
7766664

Data Quality

Data completeness: 81%