Novel Methodology for Enzymatic Removal of Atrazine from Water by CBD-Fusion Protein Immobilized on Cellulose

Abstract

s-Triazines including atrazine are heavily used agricultural herbicides, and their extensive removal from industrial wastewater is required before these effluents can be disposed. The use of enzymes for this purpose is an important potential alternative to conventional methods of detoxification. The purpose of the present study was to develop an enzyme-based technology for the treatment of atrazine contaminated water. In this paper we describe the construction and expression of two fusion proteins which dechlorinate atrazine while being firmly bound to an insoluble cellulose matrix. Dechlorination of atrazine produces in one enzymatic step the nonregulated compound hydroxyatrazine from the regulated mother compound, atrazine.

Keywords

AtrazineChemistryCelluloseEffluentDetoxification (alternative medicine)WastewaterEnzymeWater treatmentEnvironmental chemistryPulp and paper industryBiochemistryPesticideEnvironmental scienceEnvironmental engineeringAgronomyBiology

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Publication Info

Year: 2000
Type: article
Volume: 34
Issue: 7
Pages: 1292-1296
Citations: 46
Access: Closed

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APA Style

                            
                                
                                    Carl Kauffmann, 
                                
                                    Oded Shoseyov, 
                                
                                    Etai Shpigel
                                
                                et al.
                            
                            (2000). 
                            Novel Methodology for Enzymatic Removal of Atrazine from Water by CBD-Fusion Protein Immobilized on Cellulose. 
                            Environmental Science & Technology
                            , 34
                            (7)
                            , 1292-1296.
                            https://doi.org/10.1021/es990754h
                        

Identifiers

DOI: 10.1021/es990754h