Abstract
The coding region of the alpha-amylase inhibitor (HaimII) gene from the producing strain Streptomyces griseosporeus YM-25 was localized on an 800-base-pair DNA segment. The nucleotide sequence of a 1,191-base-pair region including the HaimII gene was determined by the dideoxy-chain termination method. The nucleotide sequence data predicted an open reading frame of 363 base pairs starting with an ATG initiation codon and ending with a TGA translational stop codon. The amino acid sequence deduced from the nucleotide sequence indicated that the presumptive pre-HaimII protein extends 37 amino acids to the amino terminus and 6 amino acids to the carboxyl terminus of the mature HaimII protein. The pre-HaimII protein is believed to be processed both during and after secretion. Two forms of the inhibitor, which have a higher molecular weight than that of the HaimII protein isolated from S. griseosporeus, were partially purified from the culture filtrate of Streptomyces lividans containing the cloned HaimII gene.
Keywords
BiologyNucleic acid sequenceOpen reading frameGenePeptide sequenceMolecular biologyStreptomycesStop codonCoding regionStart codonAmino acidNucleotideBiochemistryGeneticsBacteria
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Publication Info
- Year
- 1988
- Type
- article
- Volume
- 170
- Issue
- 10
- Pages
- 4451-4457
- Citations
- 26
- Access
- Closed
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Cite This
Hiroshi Nagaso,
Shin Saito,
Haruki Saito
et al.
(1988).
Nucleotide sequence and expression of a Streptomyces griseosporeus proteinaceous alpha-amylase inhibitor (HaimII) gene.
Journal of Bacteriology
, 170
(10)
, 4451-4457.
https://doi.org/10.1128/jb.170.10.4451-4457.1988
Identifiers
- DOI
- 10.1128/jb.170.10.4451-4457.1988