Abstract
On the basis of information from computer-assisted sequence comparison of the Mycoplasma pneumoniae 16S ribosomal RNA (rRNA) sequences with sequences from various other mycoplasmal and bacterial species, we constructed M. pneumoniae-specific oligonucleotide probes complementary to variable regions in the 16S rRNA molecule. Using a DNA/RNA dot blot hybridization procedure, it was possible to detect less than 1 X 10(3) mycoplasmas. This test is a most sensitive assay for species-specific detection of bacteria. It can easily be adapted for detection and identification of other bacterial species and may have wide medical and industrial application.
Keywords
16S ribosomal RNARibosomal RNAOligonucleotideBiologyDNARNAMycoplasma pneumoniaeMycoplasmaNucleic acid thermodynamicsDot blotMolecular biologyOligomer restrictionRibosomal DNABacteriaComputational biologyMicrobiologyGeneticsGenePhylogenetics
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Publication Info
- Year
- 1987
- Type
- article
- Volume
- 133
- Issue
- 7
- Pages
- 1969-1974
- Citations
- 107
- Access
- Closed
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Cite This
Ulf B. Göbel,
Andrew G. Geiser,
E J Stanbridge
(1987).
Oligonucleotide Probes Complementary to Variable Regions of Ribosomal RNA Discriminate between Mycoplasma Species.
Microbiology
, 133
(7)
, 1969-1974.
https://doi.org/10.1099/00221287-133-7-1969
Identifiers
- DOI
- 10.1099/00221287-133-7-1969