Abstract
(1) Rat cortical neurons grown in dissociated cell culture exhibit IPSPs which appear to be generated by an increase in membrane conductance to chloride. (2) The neurons are all sensitive to GABA in micromolar concentrations and GABA mimics the inhibitory transmitter. (3) The neurons are much less sensitive to glycine and insensitive to taurine. (4) Bicuculline and strychnine both block essentially all IPSPs and at the same concentrations block GABA effects. (5) It is concluded that GABA is the main, or only, inhibitory transmitter utilized by the cortical neurons in vitro. The relevance of this conclusion to in situ transmitter identification is discussed.
Keywords
Inhibitory postsynaptic potentialNeuroscienceBicucullineStrychnineGABAA receptorGlycineCortical neuronsChemistryElectrophysiologyBiologyBiophysicsBiochemistryReceptorAmino acid
MeSH Terms
AnimalsBicucullineCerebral CortexCulture TechniquesDose-Response RelationshipDrugEvoked PotentialsGlycineNeural InhibitionNeuronsPicrotoxinRatsStrychnineSynapsesgamma-Aminobutyric Acid
Affiliated Institutions
Related Publications
On the role of GABA as an inhibitory neurotransmitter in inferior colliculus neurons: iontophoretic studies
Significant neurochemical, immunocytochemical, and ligand binding studies support a role for GABA as an inhibitory neurotransmitter in the inferior colliculus (IC). The present ...
Control of cortical neuronal migration by glutamate and GABA
Neuronal migration in the cortex is controlled by the paracrine action of the classical neurotransmitters glutamate and GABA. Glutamate controls radial migration of pyramidal ne...
GABA and glycine as neurotransmitters: a brief history
γ ‐Aminobutyric acid (GABA) emerged as a potentially important brain chemical just over 50 years ago, but its significance as a neurotransmitter was not fully realized until ove...
Excitatory actions of GABA in developing rat hypothalamic neurones.
1. Gramicidin‐perforated patch clamp recording was employed to study GABA‐mediated responses in rat hypothalamic neurones (n = 102) with an intracellular Cl‐ concentration unalt...
Excitatory actions of gaba during development: the nature of the nurture
In the immature brain, GABA (gamma-aminobutyric acid) is excitatory, and GABA-releasing synapses are formed before glutamatergic contacts in a wide range of species and structur...
Publication Info
- Year
- 1980
- Type
- article
- Volume
- 190
- Issue
- 1
- Pages
- 111-121
- Citations
- 137
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
137
OpenAlex
4
Influential
112
CrossRef
Cite This
Marc A. Dichter
(1980).
Physiological identification of GABA as the inhibitory transmitter for mammalian cortical neurons in cell culture.
Brain Research
, 190
(1)
, 111-121.
https://doi.org/10.1016/0006-8993(80)91163-4
Identifiers
- DOI
- 10.1016/0006-8993(80)91163-4
- PMID
- 7378733