Abstract

The antibody content of sera from rabbits treated intermittently with free glucagon or glucagon conjugated with ovalbumin was measured at intervals during a 2-year period. Circulating nonprecipitating antibody, capable of binding glucagon-I131, was detectable within one month, and was still detectable after 4 to 5 month periods during which no antigen was administered. Glucagon-I131 injected into an immunized rabbit was rapidly bound to antibody, remained chromatographically intact and was only slowly cleared from the circulation. Glucagon-I131 could be dissociated from its antibody at acid pH. The preferential salt precipitation technic used for immunochemical assay of insulin proved applicable to measurement of minute amounts of glucagon.

Keywords

GlucagonAntibodyChemistryEndocrinologyInternal medicineImmunoassayAntigenInsulinBiologyImmunologyMedicine

Affiliated Institutions

Related Publications

Publication Info

Year
1961
Type
article
Volume
107
Issue
3
Pages
491-494
Citations
29
Access
Closed

External Links

View on DOI.org

Social Impact

Altmetric
PlumX Metrics

Social media, news, blog, policy document mentions

Citation Metrics

29
OpenAlex

Cite This

G M Grodsky, T. Hayáshida, C.T. Peng et al. (1961). Production of Glucagon Antibodies and their Role in Metabolism and Immunoassay of Glucagon.. Experimental Biology and Medicine , 107 (3) , 491-494. https://doi.org/10.3181/00379727-107-26665

Identifiers

DOI
10.3181/00379727-107-26665